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Aspartame is genetically engineered in E. coli bacteria.

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--- Quote from: David Rothscum on January 21, 2009, 05:26:33 pm ---WTF?  :o ??? ??? ???
Does anybody have any more information on this?

--- End quote ---

From Wiki:  http://en.wikipedia.org/wiki/Aspartame


Aspartame is the methyl ester of the dipeptide of the natural amino acids L-aspartic acid and L-phenylalanine. Under strongly acidic or alkaline conditions, aspartame may generate methanol by hydrolysis. Under more severe conditions, the peptide bonds are also hydrolyzed, resulting in the free amino acids.[4]

In certain markets aspartame is manufactured using a genetically modified variation of E. coli.[5][6]

I am afraid this is reaching the limits of my knowledge of Organic and Inorganic Chemistry so I can only offer tidbits.

Brevibacterium flavum
A species in the genus CORYNEBACTERIUM, family Corynebacteriaceae, which is used for industrial production of the amino acid LYSINE. It is closely related to Corynebacterium glutamicum.

Brevibacterium flavum is the strain of E. Coli bacteria that is used to culture L-aspartic acid and L-phenylalanine (Aspartame).

--- Quote ---To start the fermentation process, a sample from a pure culture of bacteria is put into a test tube containing the nutrients necessary for its growth. After this initial inoculation the bacteria begin to multiply. When their population is large enough, they are transferred to a seed tank. The bacterial strains used to make L-aspartic acid and L-phenylalanine are B. flavum and C. glutamicum respectively.

--- End quote ---

Brevibacterium flavum can also be used to culture other amino acids like Lysine and L-tryptophan[Ref.].

There are numerous references availavble (see search results here) that link Brevibacterium flavum with p-Fluorophenylalanine. Now just the sight of the prefix Fluor- should set off alarms for anyone who has researched fluoride and fluorine and well it might!

There is evidence that phenylalanine combined with p-Fluorophenylalanine is toxic. No surprise here however it is yet another piece of evidence that Aspartame is little more than a deadly poison that just so happens to taste sweet.



(From the Laboratory for the Study of Hereditary and Metabolic Disorders and the
Departments of Biological Chemistry and Medicine, University
of Utah College of Medicine, Salt Lake City, Utah)

(Received for publication, November 3, 1950)

The acute toxicity of 3-fluorotyrosine and of 3-fluorophenylalanine for
animals was reported several years ago (2) and recently it was found that 2-
and 4-fluorophenylalanines likewise are toxic when administered to albino
rats (3). 3-Fluorotyrosine and 3-fluorophenylalanine are very efficient in
acting as antimetabolites for tyrosine and phenylalanine, respectively (4),
while 4-fluorophenylalanine inhibits the growth of microorganisms (5). In
spite of the fact that tyrosine and phenylalanine were able to overcome in
a competitive manner the inhibition of growth of microorganisms caused by
3-fluorotyrosine and 3-fluorophenylalanine, it did not seem reasonable to
expect that the great toxicity’ of these derivatives to animals receiving an
adequate diet could be due to their action as antimetabolites for their parent
amino acids.

The purpose of the present investigation was to examine the effect of
phenylalanine in counteracting the toxicity of 4-fluorophenylalanine for
the rat. This study was of considerable interest because only a few of the
many known competitive antagonists for amino acids, ethionine (6-9),
methoxinine (lo), and p-2- and P-3-thienylalanines (ll-13), have been
studied to determine whether their toxic effects to animals can be removed
by the presence of an excess of the parent amino acids. In the case of the
thienylalanines the workers concluded that the toxicities could be removed
completely by the addition of an excess of the normal metabolite to the
diet. With ethionine and methoxinine, however, there is strong evidence
to indicate that factors other than a simple competitive antagonistic action
towards methionine are responsible for their toxicity to animals...

It has been found that the inhibition of the growth of rats produced by
the addition of 4-fluorophenyl-nn-alanine to their diets can be alleviated,
but not completely overcome, by the addition of relatively larger amounts
of phenylalanine to the same diets. One factor responsible for the lack of
complete reversibility of the toxic action of 4-fluorophenylalanine for animals
has been found to be the production of inorganic fluoride from the
organic fluorine-containing compound.

Suggestions have been offered to explain the different behavior observed
in microorganisms and in mammals with certain amino acid antimetabolites.
PDF LINK > http://www.jbc.org/cgi/reprint/190/2/461.pdf
--- End quote ---

Other References:

How Aspartame is made

gEEk squad:
Here is the original patent for making aspartame


Count how many times you can find the word E. Coli!


--- Quote from: gEEk squad on January 21, 2009, 07:12:04 pm ---Here is the original patent for making aspartame


Count how many times you can find the word E. Coli!

--- End quote ---
Thanks for the info.

It is not mentioned on the diet Coke bottle labels that GE is involved.

This is a quote from the link you put up under "Description."
PROCESS FOR PRODUCING ASPARTAME This invention relates to the production of artificial sweeteners and more particularly to the production of sweet tasting peptides through the use of genetically manipulated microorganisms.


--- Quote from: gEEk squad on January 21, 2009, 07:12:04 pm ---Here is the original patent for making aspartame


Count how many times you can find the word E. Coli!

--- End quote ---

3  Do I win a prize? ;D


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