Zika Virus - The New Globalist Plague Disease

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Offline KazKru

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Zika Virus - The New Globalist Plague Disease
« on: April 09, 2011, 12:10:16 AM »
http://www.foxnews.com/health/2011/04/08/man-sexually-transmits-insect-borne-disease-wife/?test=latestnews

Man Sexually Transmits Insect-Borne Disease to Wife
Published April 08, 2011| FoxNews.com

Scientists think they may have documented the first case of a sexually transmitted insect-borne disease, according to a study in Emerging Infectious Diseases.

Brian Foy, a vector biologist at Colorado State University who traveled to Senegal, was bitten by a mosquito and subsequently developed the Zika virus, which causes fatigue and joint pains.

When Foy returned to the U.S. and had sex with his wife, he unknowingly transmitted the disease to her.

........(more at link)

UPDATE to 2016 and the Zika Virus Outbreak - Brazil, other areas:
http://forum.prisonplanet.com/index.php?topic=313702.0

Offline Valerius

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Re: Man Sexually Transmits Insect-Borne Disease to Wife
« Reply #1 on: April 09, 2011, 02:01:56 AM »
I heard this.

This is really, really, bad news.
"No man can put a chain about the ankle of his fellow man without at last finding the other end fastened about his own neck."  -Frederick Douglass

Offline Dig

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    • Git Ureself Edumacated
Production of a Transgenic Mosquito Expressing
Circumsporozoite Protein, a Malarial Protein, in the Salivary
Gland of Anopheles stephensi (Diptera: Culicidae)
Hiroyuki Matsuoka*, Tsunetaka Ikezawa, and Makoto Hirai
http://www.lib.okayama-u.ac.jp/www/acta/pdf/64_4_233.pdf
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We are producing a transgenic mosquito, a flying syringe, to deliver a vaccine protein to human beings via the saliva the mosquito deposits in the skin while biting. The mosquito produces a vaccine protein in the salivary gland (SG) and deposits the protein into the hostʼs skin when it takes the hostʼs blood. We chose circumsporozoite protein (CSP), currently the most promising malaria vaccine candi- date, to be expressed in the SG of . To transform the mosquitoes, plasmid containing the CSP gene under the promoter of female SG-specific gene, as well as the green fluorescent protein (GFP) gene under the promoter of 3xP3 as a selection marker in the eyes, was injected into more than 400 eggs. As a result, five strains of GFP-expressing mosquitoes were established, and successful CSP expression in the SG was confirmed in one strain. The estimated amount of CSP in the SG of the strain was 40ng per mosquito. We allowed the CSP-expressing mosquitoes to feed on mice to induce the production of anti-CSP antibody. However, the mice did not develop anti-CSP antibody even after transgenic mosquitoes had bitten them several times. We consider that CSP in the SG was not secreted properly into the saliva. Further techniques and trials are required in order to realize vaccine-delivering mosquitoes.

Key words: malaria, salivary gland, flying syringe, transgenic mosquito, vaccineosquitoes are nuisance to human beings because they puncture the skin to obtain blood, which they use for their eggs. They deposit saliva in the skin when they take blood, and this causes itching [1]. They also transmit pathogens of diseases such as malaria, filarisis, yellow fever, and dengue fever. People have long tried to prevent mosquitoes from biting using mosquito nets [2] and repellents [3]. Moreover, people have attacked mosquitoes with insecticides to eliminate them [4, 5]. These struggles have continued not only in developing countries but also in advanced countries.

  In the last 30 years, developments in genetic engineering have allowed us to add new genes to wild-type cells or to remove protein genes from them [6]. These technologies were used at first in bacteria or yeast to obtain recombinant proteins for experiments and to understand the mechanisms of protein molecules. More recently, many transgenic experiments have been applied to plants and animals, including vertebrates and insects. Recombinant-DNA-modified soybean, cotton, and corn are widely adopted and harvested around the world [7]. Transgenic mice, sheep, dogs, zebra fish, flies, silkworms, and so on have appeared in laboratories as well [8-12]. The objectives of genetic engineering in animals are several: to understand cell differentiation, to find molecules responsible for causing specific diseases, and to speed up the production of meat, fish, and silk.

  Some attempts have been made to create transgenic mosquitoes to make them non-vectors of germs or to reduce the germsʼ transmission ability [13, 14]. Anopheline mosquitoes are the primary targets for transgenes, because they transmit malaria parasites. Some laboratories, including ours, have succeeded in producing transgenic mosquitoes with lower levels of malaria parasites in the digestive tract after blood meals on malaria-infected animals [15, 16]. The goal of those attempts is to control disease transmission through genetic modification of the mosquitoes [17].

  We here present a new attempt to produce a useful protein in the mosquito salivary gland (SG) by adding a new gene to a mosquito chromosome. When mosquitoes attach to the skin surface, they try to find blood vessels from which they can take blood. At that time, they first deposit their saliva in the skin [1]. Mosquito saliva contains many molecules, such as vasodilators, platelet inhibitors, an anesthetic substance, and so on [18]. These substances dilate the blood vessel, allowing the mosquito to insert its proboscis into the vessel. A mosquito can have a blood meal without the blood coagulating and without being noticed. On the other hand, humans develop anti- saliva protein antibodies after experiencing several mosquito bites [19, 20]. Our idea is to put a gene encoding a useful protein into a mosquito chromosome, causing it to make the useful protein in its saliva and to inject the protein, via the saliva, into animals or human beings upon blood feeding. We expect that the host would develop antibodies to the recombinant protein as a reaction. If these transgenic (TG) mosquitoes, whose saliva contains a vaccine protein against a disease, were spread in an area where people are suffering from the disease, people who are daily bitten by them would develop antibodies to the vaccine protein, ultimately vaccinating the community from the disease. In this situation, mosquitoes would play the role of vaccine deliverers [21].

  In field conditions of malaria-endemic areas, for instance, nearly 100 mosquitoes bite an individual per night, but more than 50オ of the mosquitoes are nulliparous females (non-oviposit females) [22]. Even in malaria-holoendemic areas, the percentage of mosquitoes infected with malaria parasites in the SG is less than 4オ among collected Anopheline mosquitoes [23]. Indeed, it is important to reduce malaria-infective mosquitoes, but people in malaria-endemic areas are bitten by thousands of non-infective mosquitoes each year. These non-infective mosquitoes could work as vaccine distributors.

  We discovered one molecule of a platelet inhibitor in the SG of a model mosquito, cloned the gene, and named it the Anopheline anti- platelet protein (AAPP) [24]. This molecule is expressed only in the female SG. We used the upper stream area of the AAPP gene ( ) as a promoter for the vaccine protein. For the vaccine protein, we chose circumsporozoite protein (CSP) of the malaria parasite. This molecule has been studied as a malaria vaccine candidate [25, 26]. In a rodent malaria model, a monoclonal antibody to CSP neutralizes the infectivity of sporozoite, an infective form of malaria parasite from the mosquito [27], and a synthetic peptide induces sufficient antibodies to protect immunized mice from sporozoite challenge [28]. CSP is now thought to be a promising vaccine candidate molecule [29], and recombinant proteins composed of CSP are being evaluated in malaria-endemic areas [30]. Thus, we decided to produce a TG mosquito containing the promoter area of followed by the CSP gene ( ). We expected that the TG mosquitoes would express CSP in the SG and inject the recombinant CSP (rCSP) into the skin of mice when the TG mosquitoes fed on them.

Materials and Methods
 
SDA 500 strain was reared in our laboratory under conditions of 26℃ room temperature, 50-70オ relative humidity, and light control of 14 h bright and 10 hours dark. Female BALB/c mice were purchased from SLC (Shizuoka, Japan). A rodent malaria parasite, ANKA strain, was maintained by cyclical passage through BALB/c mice and mosquitoes.
 
The rPbCSP was expressed as His-tagged at the C-terminal by the baculovirus expression system, and purified with a nickel column (Qiagen, Hilden, Germany) as described elsewhere [31]. The rPbCSP is expressed with two molecular weight sizes, 70 and 82kDa [31]. When mice are immunized with rPbCSP three times, they become protected against parasite challenge (unpublished data). The protein concentration of rPbCSP was measured using a Bio- Rad Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA). Bovine serum immunoglobulin was used as a standard for the protein concentration.
 
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For anti-CSP antibody production, 0.5ml of rPbCSP solution (50 g/ml) and 0.5ml of alum, Al(OH)3 (16mg/ml), were well mixed and 0.2ml was injected intraperitoneally into each of four BALB/c mice. Injection was done 3 times at intervals of 2 weeks. Anti-CSP antibody was monitored by ELISA, as shown below, and the mice were sacrificed to taketheir sera. The sera were used as the anti-CSP antibody.
 
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To prepare anti-saliva antibody, 4 female BALB/c mice were each bitten by 100 female mosquitoes every 2 weeks for a 10-week period (5 biting sessions). Development of an anti-saliva antibody in the mice was confirmed by ELISA as shown below, and sera were taken from the mice. The sera were used as the anti- saliva antibody.
 
   For the evaluation of anti-CSP antibody, 8 wells of a 96-well assay plate were coated with 100 l of rPbCSP (1 g/ml) in 0.05M carbonate buffer (pH9.6), and 8 wells were filled with 100 l of carbonate buffer without antigen. The plate was incubated at 4℃ overnight.

  For the evaluation of anti-saliva antibody, 10 pairs of SG were collected from female mosquitoes, destroyed in 1.0ml of carbonate buffer by sonication (1 sec×5 times), and centrifuged (8,000rpm for 3min). The supernatant was used as the SG antigen. Eight wells were each coated with 100 l of SG antigen.

  Two microliters of blood was collected from the tail of each mouse 7 days after the injection of rPbCSP or mosquito biting. The blood was mixed with 0.8ml of phosphate-buffered saline containing 1オ bovine serum albumin (BSA/PBS) and centrifuged at 8,000rpm for 3min. The supernatant was used as an 800-fold-diluted serum.

  After the antigen solution was removed and washed twice, the wells of an ELISA plate were blocked with 150 l of 1オ BSA/PBS for 30min. After removing the blocking solution, 100 l of the mouse serum diluted 800-fold was distributed into each of 2 antigen wells and 2 no-antigen wells and incubated for 2h. As the second antibody, rabbit anti-mouse IgG conjugated with horseradish peroxidase (HRP) (Bio-Rad Laboratories) diluted 3,000-fold was distributed into each of the same wells and incubated for 1h. After the plate was washed, the substrate solution, a mixture of 0.04オ 2,2ʼ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich, St. Louis, MO, USA), 0.05オ H2O2 in 0.05M phosphate, and 0.1M citrate buffer (pH4.5) was added. After 20-60min, a green color appeared. The absorptionwas measured at a wavelength of 405 nm by a microplate reader, Spectra Max M5 (Molecular Devices, Tokyo, Japan). The mean absorbance of antigen wells minus the mean absorbance of no-antigen wells was taken as the OD value for the antigen.
 
The anopheline anti-platelet protein gene ( ) promoter region and its signal peptide coding region of  [24] (1,746bp) were amplified with a primer pair of AAPP-F-(5ʼ-TTATAAGACGGAGCTCATTGTCGCTCGTC-3ʼ) and AAPP-R-(5ʼ-CGGCCGTGCGGATACGATCAGCGCAAGGC-3ʼ), then cloned into the pCR-BluntII TOPO vector (Invitrogen, Carlsbad, CA, USA) (plasmid a).

The open reading frame of the gene without a signal peptide coding region or a C-terminal GPI-anchor domain coding region was amplified with a primer pair of PbCS-F-(5ʼ-CAAAATAAAATCATCCAAGCCCAAAGGAAC-3ʼ) and PbCS-R-(5ʼ-TATTTATCCATTTTACAAATTTCAGTATCAATATC-3ʼ), then cloned into the vector (plasmid b). The 3ʼ non-coding region of the AAPP gene was amplified with a primer pair of 3UTR-F-(5ʼ-GAAACACACCGTTAACGACAC-3ʼ) and 3UTR-R-(5ʼ-TATTCAAAGGTCCACAAATGTC-3ʼ), then cloned into the vector (plasmid c). The inserts of plasmids a, b, and c were serially cloned into the pENTR4 vector (Invitrogen) (donor vector).

  The pBac [3xP3-EGFP] vector [11] and helper plasmid were kindly provided by Professor A. S. Raikhel (Department of Entomology, University of California at Riverside, USA). The pBac [3xP3-EGFP] vector was digested with , blunted with a Klenow fragment, and dephosphorylated. The reading frame cassette A (Invitrogen) was then cloned into the vector (destination vector). The insert in the donor vector was cloned into the destination vector by Gateway LR clonase reaction (Invitrogen). The resulting plasmid (pBac-AAPP-PbCSP: Fig. 1A) was expected to drive CSP gene expression under the AAPP promoter. The pBac-AAPP-PbCSP plasmid was mixed with piggyBac helper (Fig. 1B) and micro-injected into the eggs of as described below.
 
The mosquito microinjection was performed as described elsewhere [32]. In brief, blood-fed mosquitoes were allowed to lay eggs on a wet filter sheet 72-84h after a blood meal. Eggs were laid and injected with plasmids within 90min after oviposition. Injection was done by glass needles (Eppendorf, Hamburg, Germany) with a mixture of the pBac-AAPP-PbCSP (500ng/ l) and piggyBac helper (300ng/ l) in injection buffer (5mM KCl, 0.1mM Na2HPO4, pH6.8). After injection, the eggs were placed in water and observed for hatching. Hatched larvae were analyzed on a fluorescence microscope at a wavelength of 490nm to detect GFP expression.
 
We selected GFP-expressing larvae and made them emerge (G0). One G0 adult female (or male) expressing GFP was put in a cage containing 5 wild-type males (or females). After mating and blood feeding, each female was allowed to lay eggs individually. Hatched larvae were observed under a fluorescence microscope, and GFP-expressing larvae were isolated as a G1 strain. Among the same batch of G1, mosquitoes were allowed to mate, feed, and lay eggs. Hatched larvae were observed and GFP-expressing larvae were isolated as the G2 of the strain.
 
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Ten pairs of SG were collected from G2 female adults in each strain, destroyed in 1.0ml of carbonate buffer by sonication (1 sec×5 times), and centrifuged (8,000rpm for 3min). The supernatant was used as the SG antigen. Then, 100 l of SG antigen was distributed into each of four wells of an ELISA plate and allowed to stand at 4℃ overnight. After blocking with 1オ BSA/PBS, anti-CSP antibody diluted 800-fold was poured into 2 wells, and normal mouse serum diluted 800-fold was poured into 2 other wells. Incubation with the secondary antibody and the subsequent proce- dures were the same as described above.
 
Ten pairs of salivary glands were collected from both TG female mosquitoes and wild-type female mosquitoes. Samples were separated on a 10オ SDS-polyacrylamide gel under reducing conditions (with 2オ 2-mercaptoethanol), transferred onto a nitrocellulose (NC) sheet, and probed with mouse anti-CSP antibody diluted 800-fold. The NC sheet was next incubated with anti-mouse IgG conjugated with HRP (Bio-Rad Laboratories) diluted 3,000 fold, then reacted with substrate, SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Rockford, IL, USA). Positive bands were visualized in a Lumino Image Analyzer (LAS-1000) (Fuji Film, Tokyo, Japan).
 
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Two female BALB/c mice were bitten by each of 50 TG female mosquitoes every 2 weeks over a 10-week period (5 biting sessions). Two other mice were bitten by wild- type female mosquitoes under the same protocol. Two microliters of mouse blood was collected from the tail vein 7 days after the final mosquito biting. Antibody assay to CSP and SG was carried out by ELISA as described above.

  A pair of SG of the TG female mosquitoes before blood feeding was trans- ferred in 0.5ml of carbonate buffer, destroyed, and centrifuged as above. Another TG female mosquito was dissected within 10min after full blood feeding, and a pair of SG were isolated and treated as described above. Each antigen solution was added to 4 wells, 100 l per well. Anti-CSP antibody (800-fold) was reacted with the 2 wells and anti-saliva antibody (800-fold) was reacted with the other 2 wells. Incubation with the secondary antibody and the subsequent procedures were the same as described above. Eight SG samples in each group were tested.

Results and Discussion
 
We injected recombinant plasmid into 461 mosquito eggs, from which 109 larvae hatched and 103 adults emerged (G0 adults: 56 males and 47 females). We crossed these G0 males with wild-type females and allowed them to lay eggs. We also crossed G0 females with wild-type males and allowed them to lay eggs. GFP-expressing larvae were selected and allowed to emerge. From the G0 male group we established 1 strain, and from the G0 female group we established 4 strains. The rate of establishment for 5 strains from the 461 egg injections (1.1オ) was reasonable compared with our previous experiments [16, 33] and with the results obtained by other groups [15, 32].
  As shown in Fig. 2, larvae and adult TG mosquitoes expressed GFP in the eyes as expected, because the promoter works specifically in the eyes [11]. Unexpectedly, GFP was expressed not only in the eyes but also in the SG (Fig. 2C). 3xP3 marker protein is typically used for (a small fly) transgenesis, and expression of a transgene in the SG of has not been confirmed. Thus, this is the first observation that the promoter works in the SG of a mosquito. The SG cells may contain some transcriptional factors to attach to the promoter. GFP expression in female SG was observed among all strains of CS1 to CS5. In the SG, the highest expression of GFP was observed in the distal region of the lateral lobes in all SGs (Fig. 2C). In contrast, GFP was not always expressed in the median lobes. This tendency was similar to the results of a previous report [33].
 
We expected that rCSP would be expressed in the SG of TG mosquitoes. ELISA was performed to confirm rCSP expression. Ten pairs of SG from each strain of TG female mosquitoes were collected and used as ELISA antigens. Only one of the 5 strains (CS2) showed strong reactivity against anti-CSP antibody (Fig. 3). This means that the promoter worked in all 5 strains, but that the promoter worked only in strain CS2. The exact reason for this is unknown, but may be related to the insertion site in the mosquito chromosome. The insertion site may allow the promoter, but not the promoter, to work. A possible explanation for this is that some of the transcriptional factors that attach to the  promoter to start AAPP expression react mostly with the intrinsic promoter. The inserted promoter cannot react with these factors, and thus rCSP may be expressed in only small amounts.
 
Western blotting was performed with 10 pairs of SG dissected from transgenic mosquitoes of the CS2 strain. A band of 38kDa reacted with anti-CSP in the TG mosquito lane (Fig. 4). The molecular size was as we expected for rCSP. This indicates that we succeeded in producing a for- eign protein of rCSP in the mosquito SG. A comparison of the density of the 38kDa band with those of the positive control bands of a series of different amounts of rPbCSP revealed that the amount of rCSP in the CS2 lane was equivalent (in reactivity with the anti-CSP antibody) to 400ng rPbCSP. This, in turn, indicated that approximately 40ng of rCSP was expressed in one pair of SG in the TG mosquito.

  Besides the 38kDa band, some other faint bands appeared in the CS2 lane. The molecular sizes of these bands were 26, 48, 64, and 80kDa. Even in the reducing conditions (with 2オ 2-mercaptoethanol), some intermolecular re-binding may have occurred. There is another possibility that rCSP binds to some molecules of the SG components such as ubiquitins, proteosome-associated cofactors [34].
 
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Two Female BALB/c mice were each bitten by 50 TG female mosquitoes of the CS2 strain every 2 weeks over a 10-week period. The mice developed anti-SG antibody but did not develop anti- CSP antibody (Fig. 5). In our prediction, 20オ of rCSP (about 8ng of rCSP per mosquito) in the SG should be injected into a mouse during a single blood feeding. In each blood feeding, a mouse received 50 TG mosquito bites and received a total of 400ng of rCSP in the skin. As these blood feedings were conducted 5 times, each mouse was injected with 2,000ng of rCSP. From these injections, the mice should have developed anti-CSP antibody in the serum. However, no antibody against rPbCSP was detected in the mice. Only anti-SG antibody was detected in the mouse serum. In our experience, 2 injections each of 1,000ng rPbCSP are sufficient to develop anti- rPbCSP in mice (unpublished observation). Thus, we consider the possibility that rCSP was not secreted as we had expected in the saliva of the TG mosquito and thus was not injected into the skin during blood feeding.

We performed ELISA with antigens of the SG before and after blood feeding. The amount of rCSP in the SG after blood feeding did not decrease, although the amount of total saliva protein did decrease (Fig. 6). This indicates that the TG mosquitoes consume a lot of saliva during blood feeding and that the saliva does not contain rCSP.

  We used the promoter for rCSP expression because AAPP was confirmed to be secreted in the saliva and injected into the skin during blood feeding [24]. However, we obtained the disappointing result that the TG mosquito did not properly release the foreign protein into the mouse skin. One possible explanation is that rCSP may have been captured by some components of the SG cells, such as ubiquitins [35]. As shown in Fig. 4, rCSP was expressed as some larger molecules in Western blot analysis. This figure suggests the possibility of “captured rCSP” in the SG. According to this hypothesis, rCSP cannot be secreted in the saliva because of its molecular characteristics, even if it has a secretion signal. We are preparing a new construct using the promoter and secretion signal with other foreign protein genes to test this hypothesis.

  In summary, we presented a transgenic mosquito expressing a foreign protein in the SG. The expressed protein was rCSP, a promising malaria vaccine candi- date protein. The amount of rCSP in one pair of SG was estimated to be 40ng. If 20オ of rCSP in the SG is injected into a mouse during a single blood feeding band there are 50 mosquito bites on the mouse, 8× 50ng of CSP should be injected and anti-CSP antibody should be developed in the mouse after several episodes of blood feeding. Since we did not succeed in preparing such a TG mosquito, we will develop new constructs for producing alternative TG mosquitoes. Acknowledgments. This work was supported by a grant from the Bill & Melinda Gates Foundation through the Grand Challenges Exploration Initiative to HM as well as by a grant-in-aid from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (16590344 to HM). Some items of the experimental apparatus were subsidized by JKA through its promotional funds from Keirin Racing.

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Offline larsonstdoc

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Zika Virus fight -- BRAZIL--200,000 Soldiers Sent House to House
« Reply #3 on: January 26, 2016, 03:31:53 PM »
http://www.dailymail.co.uk/news/article-3417175/Brazil-sends-200-000-soldiers-stop-spread-Zika-virus-outbreak-seen-huge-numbers-babies-born-small-heads.html

Brazil sends in 200,000 soldiers to stop the spread of the Zika virus outbreak which has seen huge numbers of babies born with small heads and cast a shadow over the Olympics

More than 200,000 soldiers are being sent 'house to house' in Brazil in the battle against Zika-carrying mosquitoes

They are to distribute leaflets and dispense advice, signalling a major ramping up of efforts against the Zika virus

Although not deadly, the virus has been linked to cases of severe brain damage and birth defects in newborn babies

Pregnant women are being told to avoid travelling to the affected 22 countries, including in Latin America and Africa

Cases have also been reported in Europe, with four in Italy, three in Britain and two in region of Catalonia in Spain


2011 Background:
http://forum.prisonplanet.com/index.php?topic=205602.msg1229189#msg1229189
I'M A DEPLORABLE KNUCKLEHEAD THAT SUPPORTS PRESIDENT TRUMP.  MAY GOD BLESS HIM AND KEEP HIM SAFE.

Offline larsonstdoc

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I'M A DEPLORABLE KNUCKLEHEAD THAT SUPPORTS PRESIDENT TRUMP.  MAY GOD BLESS HIM AND KEEP HIM SAFE.

Offline larsonstdoc

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I'M A DEPLORABLE KNUCKLEHEAD THAT SUPPORTS PRESIDENT TRUMP.  MAY GOD BLESS HIM AND KEEP HIM SAFE.

Offline larsonstdoc

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I'M A DEPLORABLE KNUCKLEHEAD THAT SUPPORTS PRESIDENT TRUMP.  MAY GOD BLESS HIM AND KEEP HIM SAFE.

Offline chris jones

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  The CDC has known about this virus for decades,
The Zika virus — where did it come from and where will it end?
www.ft.com › World - It was first isolated in 1947 from a rhesus monkey in Uganda's Zika forest. ... Zika only began to arouse serious concern among global health  ...
  Aids, Ebola, Swine Flu, etc, etc.
We are told there is no vacine for ZIKA, there will be and bigpharma $$ as does global population control, it's not a coin toss, it is a win, win, win for the globalists.
 A vacine will be developed, another soft kill. The CDC has known of this for decades yet did nothing, I have to ponder on that, they discovered it, isolated it, defined it over one half century ago then what**-it has become global.                 Just my opinion.
Brazil is up sh**ts creek  Olympics 2016  sincerely-( my heart goes out to the mothers and the children, family's effected)..
 

Offline John_Back_From_The_Club_O

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Bill Gates mosquitoes?
The Crowd Shouted... “Give us Barabbas!” ... and People, The NWO Gave Him To You.
http://www.dominicanajournal.org/give-us-barabbas/

https://www.greatagain.gov

Offline chris jones

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Bill Gates mosquitoes?
           Sure, billy boy is in with the pack...These fmuckers consider human beings a virus in their garden.
The King;
https://www.youtube.com/watch?v=taoHk_enqWA 2012 -
His Royal Virus Prince Philip in 1984 - Human Population Reaching Plague Proportions. ... The proponents of Darwinian eugenics programs, like the British .... Stop breeding as you say there are too many human beings on our planet. .... Britain's Nazi King - Duration: 45:50. yeoldbasser 202,020 views.

  Just tossing it in there for a  example of the elites mindset.... Why go nuclear and damage their green, uh uh, there are other ways to depopulate..THIS IS BUT ONE OF THEM!!!!
  Nukes, they may light up a few to create chaos, numb the minds of the masses and divert their true methods of population reduction.

Offline donnay

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Bill Gates mosquitoes?

No doubt in my mind.  These evil pukes will stop at nothing to achieve what they want.   >:( >:(

Please visit my website: https://www.theherbsofthefield.com/

Offline chris jones

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  Viruse's, vacines, CDC, World Health Org, AMA,etc...., on the elites  pad..

Offline jofortruth

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Is Zika Virus connected? Dr Francis Boyle says on show today, "Yes" he thinks it's a bio weapon straight out of the Bill Gates Fdn:

http://z4.invisionfree.com/The_Great_Deception/index.php?showtopic=12124&st=0#entry22048022
Don't believe me. Look it up yourself!

Offline John_Back_From_The_Club_O

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Is Zika Virus connected? Dr Francis Boyle says on show today, "Yes" he thinks it's a bio weapon straight out of the Bill Gates Fdn:

http://z4.invisionfree.com/The_Great_Deception/index.php?showtopic=12124&st=0#entry22048022

Both Bill and Melinda need to stand before a 'REAL' tribunal.  These are the people YOU trust to inject you, your children with vaccine crapola?
The Crowd Shouted... “Give us Barabbas!” ... and People, The NWO Gave Him To You.
http://www.dominicanajournal.org/give-us-barabbas/

https://www.greatagain.gov

Offline jofortruth

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Don't believe me. Look it up yourself!

Offline jofortruth

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Don't believe me. Look it up yourself!

Offline jofortruth

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Don't believe me. Look it up yourself!

Offline Geniocrat

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Re: Zika Virus fight -- BRAZIL--200,000 Soldiers Sent House to House
« Reply #17 on: January 29, 2016, 05:03:03 PM »
The Nefilim could operate in the jungles of Brazil free from any human spy agency.....

Very curious....

Offline Geniocrat

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I guess AIDS didn't yield the results the Nefilim were looking for so they are trying something new.

Amazing how no matter how bad the world-wide depression gets the Nefilim run global banks never seem to deplete their eugenics funds.

Rule by consensus; NOT Homo Capensis !

Offline jofortruth

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Re: Zika Virus fight -- BRAZIL--200,000 Soldiers Sent House to House
« Reply #19 on: January 29, 2016, 05:21:51 PM »
Panic on SECOND American Airlines flight as mystery fainting illness hits passengers and crew
http://www.mirror.co.uk/news/world-news/panic-second-american-airlines-flight-7272258


The American Airlines flight, which was flying from Brazil to the United States
Don't believe me. Look it up yourself!

Offline jofortruth

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Beware the new 'Breakthrough' Transgenic Mosquitoes
http://www.i-sis.org.uk/Beware_the_New_Bre..._Mosquitoes.php

Quote
A good trick but no consideration of risks

A team led by Andrea Crisanti at Imperial College London in the UK was widely reported to have made a breakthrough or even a ‘quantum leap’ in creating transgenic mosquitoes that could eradicate malaria [1]. Unfortunately, it is potentially the most hazardous genetically modified organism (GMO) to have been created, and should go no further from the laboratory. The researchers have not considered the risks involved, which would have been obvious from a casual review of existing literature.

--

To conclude

Transgenic mosquitoes are not the solution to eradicating dengue or malaria. On the contrary, they are among the most hazardous GMOs created, and should never be released into the wild on any commercial basis.
Don't believe me. Look it up yourself!

Offline chris jones

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Genetically Modified Mosquitoes Released in Brazil in 2015 ...
www.globalresearch.ca/genetically-modified-mosquit... - Traduzir esta página
 - Genetically Modified Mosquitoes Released in Brazil in 2015 Linked to the Current Zika Epidemic? By Reddit. Global Research, January 29, ...

Offline chris jones

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Re: Zika Virus fight -- BRAZIL--200,000 Soldiers Sent House to House
« Reply #22 on: January 30, 2016, 08:02:01 PM »
Genetically Modified Mosquitoes Released in Brazil in 2015 ...
www.globalresearch.ca/genetically-modified-mosquit... - Traduzir esta página
1 dia atrás - Genetically Modified Mosquitoes Released in Brazil in 2015 Linked to the Current Zika Epidemic? By Reddit. Global Research, January 29, ...
  Jo. I am convinced the elites favorite depopulation method is biowarfare. Hollywood plays into the apocolyptic nuke fest, i don't buy into that sh**t. They want us dead not their green garden  or wildlife.I have searched and find no case of an animal being effected by this, just us lil peon humans.
 

Offline SpaceCommand

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More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #23 on: February 01, 2016, 12:08:22 AM »
http://www.atcc.org/Products/All/VR-84.aspx?slp=1&geo_country=us#history

Zika virus (ATCC® VR-84™)

Name of Depositor    J. Casals, Rockefeller Foundation

Source Blood from experimental forest sentinel rhesus monkey, Uganda, 1947
Year of Origin    1947
References    

Dick GW. Trans. R. Soc. Trop. Med. Hyg. 46: 509, 1952.
And yet the same revolutionary beliefs for which our forebears fought are still at issue around the globe—the belief that the rights of man come not from the generosity of the state, but from the hand of God.

John F. Kennedy Inaugural Address

Offline John_Back_From_The_Club_O

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #24 on: February 01, 2016, 12:39:54 AM »
http://www.atcc.org/Products/All/VR-84.aspx?slp=1&geo_country=us#history

Zika virus (ATCC® VR-84™)

Name of Depositor    J. Casals, Rockefeller Foundation

Source Blood from experimental forest sentinel rhesus monkey, Uganda, 1947
Year of Origin    1947
References    

Dick GW. Trans. R. Soc. Trop. Med. Hyg. 46: 509, 1952.

Ah, but this virus has been GMO-ed and spiced into a GMO-ed mosquito.  Translation:  This ain't you're grand daddy Rockefeller's virus.
The Crowd Shouted... “Give us Barabbas!” ... and People, The NWO Gave Him To You.
http://www.dominicanajournal.org/give-us-barabbas/

https://www.greatagain.gov

Offline One Revelator

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #25 on: February 01, 2016, 01:19:53 AM »
Can't tell which is more disturbing. The “add to cart” button or the fact that they've patented, trademarked, and kept this in cold storage for 69 years and counting.

I guess being an old 1st generation virus brings the price down to a mere $430.

Plus shipping.

Worldwide.

But.....since it's owned and patented by the Rockefeller Foundation, doesn't that create legal liability in the event of some outbreak?
The number one cause of all human poverty, misery, and death is not global warming. It’s GLOBAL LYING.

Offline Jackson Holly

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #26 on: February 01, 2016, 06:57:06 AM »


... it's the for 'profit' or 'non-profit' button that disturbs me.
St. Augustine: “The truth is like a lion; you don't have to defend it.
Let it loose; it will defend itself."

Offline Geniocrat

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #27 on: February 01, 2016, 12:58:12 PM »
Amazing.... A debt free money system used for eugenic operations but debt everywhere else.

My God what will it take....

Offline donnay

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #28 on: February 01, 2016, 02:32:21 PM »
Can't tell which is more disturbing. The “add to cart” button or the fact that they've patented, trademarked, and kept this in cold storage for 69 years and counting.

I guess being an old 1st generation virus brings the price down to a mere $430.

Plus shipping.

Worldwide.

But.....since it's owned and patented by the Rockefeller Foundation, doesn't that create legal liability in the event of some outbreak?

One would think so. Good point.
Please visit my website: https://www.theherbsofthefield.com/

Offline windyacres

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #29 on: February 02, 2016, 02:04:54 AM »
Good find, Space Command.
Be Prepared

Offline Jackson Holly

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Re: More Globalist Insanity - You can buy the Zika Virus Online!
« Reply #30 on: February 02, 2016, 07:16:48 AM »

INFOWARS.COM

Top Expert: Zika is Biowarfare
https://www.youtube.com/watch?v=oBWouNjoWOI

Published on Jan 29, 2016
International law professor Francis A. Boyle breaks
down the federal government's involvement in
biowarfare and terrorism.
St. Augustine: “The truth is like a lion; you don't have to defend it.
Let it loose; it will defend itself."

Offline jofortruth

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First Case Of Sexually Transmitted Zika Virus Confirmed In Texas
http://forum.prisonplanet.com/index.php?topic=16633.msg1586503;topicseen#msg1586503
Don't believe me. Look it up yourself!

Offline John_Back_From_The_Club_O

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The Crowd Shouted... “Give us Barabbas!” ... and People, The NWO Gave Him To You.
http://www.dominicanajournal.org/give-us-barabbas/

https://www.greatagain.gov

Offline John_Back_From_The_Club_O

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INSERT LAUGH TRACK

Ebola Vaccine Pioneer Joked About Using Bioweapons to Cull Human Population [VIDEO]

EVER NOTICE IT'S ALWAYS PEOPLE INVOLVED WITH 'VACCINE DEVELOPMENT' WHO TALK ABOUT 'CULLING THE PLANET'.
The Crowd Shouted... “Give us Barabbas!” ... and People, The NWO Gave Him To You.
http://www.dominicanajournal.org/give-us-barabbas/

https://www.greatagain.gov

Offline Jackson Holly

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    • JACKSON HOLLY'S OLD HOME PLACE
forced innoculation

forced abortion
St. Augustine: “The truth is like a lion; you don't have to defend it.
Let it loose; it will defend itself."

Offline TahoeBlue

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Re: Zika Virus fight -- BRAZIL--200,000 Soldiers Sent House to House
« Reply #35 on: February 03, 2016, 12:21:13 PM »
bump for merge
Behold, happy is the man whom God correcteth: therefore despise not thou the chastening of the Almighty: For he maketh sore, and bindeth up: he woundeth, and his hands make whole ; He shall deliver thee in six troubles: yea, in seven there shall no evil touch thee. - Job 5

Offline TahoeBlue

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http://investmentwatchblog.com/zika-virus-atcc-vr-84-the-zika-virus-was-patented-in-1947-by-the-rockefeller/
Zika virus (ATCC® VR-84™) The Zika virus was patented in 1947 by the Rockefeller

The Zika virus was patented in 1947 by the Rockefeller

http://www.lgcstandards-atcc.org/products/all/VR-84.aspx?geo_country=es#history

Name of Depositor
J. Casals, Rockefeller Foundation 


Source
Blood from experimental forest sentinel rhesus monkey, Uganda, 1947 

Year of Origin  1947 

References

Dick GW. Trans. R. Soc. Trop. Med. Hyg. 46: 509, 1952
 
| - - - -

http://www.conspiracyclub.co/2016/02/02/zika-virus-rockefeller/
The “Zika” Virus Was Created and Patented By The Rockefeller’s, The goal Is to kill Millions of People
By conspiracyclub - Feb 2, 2016



  Zika virus (ATCC® VR-84 ™) was patented, yes, patented in 1947 by the Rockefeller Foundation, one of the families more interested in manipulating power even in scientific medicine to study in universities to train future professionals who unknowingly diagnose and prescribe as learned bases.

| - - - -

https://www.youtube.com/watch?v=CE5pvrJzhKQ
Mosquito my a**: Zika Virus® was PATENTED by Rockefeller Foundation in 1947.
Behold, happy is the man whom God correcteth: therefore despise not thou the chastening of the Almighty: For he maketh sore, and bindeth up: he woundeth, and his hands make whole ; He shall deliver thee in six troubles: yea, in seven there shall no evil touch thee. - Job 5

Offline jofortruth

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WORLD PANICS OVER ZIKA VIRUS - GMO mosquitos released in areas now seeing Zika outbreak
http://www.infowars.com/world-panics-over-zika-virus/


Oxford spinout Oxitec sold to Intrexon Corporation for $160 million
http://isis-innovation.com/news/oxford-spi...or-160-million/

Fidelity has equity ownership:
http://investors.morningstar.com/ownership...view.html?t=XON

Quote
Oxitec was named a World Economic Forum technology pioneer in 2008 and has been recognised by the Bill and Melinda Gates Foundation ‘Grand Challenges for Global Health Initiative’.

Oxitec's genetically-modified mosquitos: in the public interest?
http://www.councilforresponsiblegenetics.o.../I5G5KXD2L3.pdf

Quote
Bottom Pg 2: "Conditional lethality means that the mosquitos have been engineered to be able to survive to adulthood only in the presence of tetracycline (an antibiotic used to treat bacterial infections). GM mosquitos are bred to adulthood in the lab in the presence of the antibiotic and males are then released into the environment."

Dr. Group on the Alex Jones show 2-3-16 (Dr Group Begins 40 mins into the 3rd hour)said "Brazil has the highest levels of Tetracycline". He also mentioned the Summer olympics are in Brazil this Summer  and that "these mosquitos are vectors like a vaccine". Coincidence?)

http://cdn1.gcnlive.com/cache/gcn_archives...4hour=0&4date=0
Don't believe me. Look it up yourself!

Offline John_Back_From_The_Club_O

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Zika Virus Hoax. Absence of Evidence
« Reply #38 on: February 03, 2016, 11:03:12 PM »
I just want to remind the readers that when the 'so-called' Disney measles outbreak occurred, it was coincidently at the very same time the famous CDC whistleblower was going to give damning congressional testimony that the CDC knew all along that not only does big pharma know vaccines cause autism but they do everything they can to hide any and all evidence.  I never believed for a minute that there ever was a 'real' measles out break. Not even for a second.
--------------------------------------------
By Jon Rappoport

Here are five things that will happen next.

One: Health agencies and reporters will mention cases of microcephaly in other countries, and they will automatically connect them to the Zika virus, or they will suggest there could be a connection. This baseless claim is part of the operation to build up the story and spread fear. Microcephaly can be caused by any insult to the brain during fetal development.

Two: There will be more stories about the rush to develop a vaccine against Zika, the virus that hasn’t been proved to cause anything serious.

Three: Some independent researchers will continue to insist that Zika is actually a weaponized biowar virus. They will ignore the fact that, as yet, Zika hasn’t been shown to cause microcephaly. Or they will point to genetically engineered mosquitoes and the Tdap vaccine as the cause of the Zika epidemic—when there is no proof the epidemic exists. Yes, the vaccine and the mosquitoes are quite dangerous to health, but there is no reason to tout a Zika/microcephaly epidemic when proof isn’t there.

Four: The truth here is: absence of evidence. On the one hand, the CDC and the World Health Organization will grudgingly admit it would be useful to assemble more hard evidence connecting Zika to microcephaly. On the other hand, they will press forward with emergency warnings to pregnant mothers; travel advisories; and they will emphasize the need to come up with a vaccine. Then, they’ll forget all about the need for more evidence.

Five: Investigators and researchers will ignore the fact that there is a very real health crisis in Brazil, and it has existed for a long time. The rampant use of toxic pesticides, grinding poverty, contaminated water, lack of basic sanitation, overcrowding, the takeover of farm land by major corporations, prior toxic vaccine campaigns—these are all factors that cause massive illness, suffering, and death in Brazil.

FULL ARTICLE
The Crowd Shouted... “Give us Barabbas!” ... and People, The NWO Gave Him To You.
http://www.dominicanajournal.org/give-us-barabbas/

https://www.greatagain.gov

Offline jofortruth

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Re: Zika Virus Hoax. Absence of Evidence
« Reply #39 on: February 04, 2016, 08:42:02 AM »
There is also an overuse of the antibiotic Tetracycline in Brazil (Dr Group mentioned this on show yesterday - See following) We all know what an overuse of Antibiotics causes. Also, the Zika virus Oxitech technology is "engineered to be able to survive to adulthood only in the presence of tetracycline" Now isn't that interesting! (Read the PDF at this link pg 2): (I think there is indeed something going on here, but it may not be what we now see, but could be far more sinister in the long run if its about depopulation or mass immunization both of which are highly suspect due to Bill Gates comments over time. In the interview with Prof Boyle, he said not to trust the WHO, CDC or any of their buddies. They seem to be fearmongering on one hand and not telling us the truth about what is really going on, on the other hand.)
http://forum.prisonplanet.com/index.php?topic=205602.msg1586592#new

http://www.mayoclinic.org/healthy-lifestyle/consumer-health/in-depth/antibiotics/art-20045720

Don't believe me. Look it up yourself!